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anion exchange chromatography  (Bio-Rad)


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    Structured Review

    Bio-Rad anion exchange chromatography
    Anion Exchange Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anion+exchange+chromatography/Protein+Standard+for+Anion+Exchange+Chromatography/10__1016_slash_j__livres__2026__04__001-46-46-58
    Average 94 stars, based on 299 article reviews
    anion exchange chromatography - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Calreticulin-Mediated Quality Control of the Non-Classical MHC-I Molecule MICA: Implications for Immune Surveillance
    Article Snippet: Human CRT, coding for residues 18–417, was cloned into the pET-15b vector (Novagen, Madison, WI, USA). .. Both vectors were transformed in E. coli BL21 DE3 and purified in agarose Ni-NTA columns (Invitrogen, Carlsbad, CA, USA) and via anion exchange chromatography (UNO Q-6 Biochromatography column, Bio-Rad, Hercules, CA, USA), as previously described [ , ]. ..

    Article Title: Calreticulin-Mediated Quality Control of the Non-Classical MHC-I Molecule MICA: Implications for Immune Surveillance.
    Article Snippet: Human CRT, coding for residues 18–417, was cloned into the pET-15b vector (Novagen, Madison, WI, USA). .. Both vectors were transformed in E. coli BL21 DE3 and purified in agarose Ni-NTA columns (Invitrogen, Carlsbad, CA, USA) and via anion exchange chromatography (UNO Q-6 Biochromatography column, Bio-Rad, Hercules, CA, USA), as previously described [35,36]. ..

    Purification:

    Article Title: Calreticulin-Mediated Quality Control of the Non-Classical MHC-I Molecule MICA: Implications for Immune Surveillance
    Article Snippet: Human CRT, coding for residues 18–417, was cloned into the pET-15b vector (Novagen, Madison, WI, USA). .. Both vectors were transformed in E. coli BL21 DE3 and purified in agarose Ni-NTA columns (Invitrogen, Carlsbad, CA, USA) and via anion exchange chromatography (UNO Q-6 Biochromatography column, Bio-Rad, Hercules, CA, USA), as previously described [ , ]. ..

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Cell numbers were determined using the Countess II FL automated cell counter (Life Technologies). .. The human leucyl transfer RNA fused hsa-pre-miR-34a carrier-based miR-148a-3p (BioRNALeu/miR-148a-3p) (Fig. 1A) and the corresponding control RNA (BioRNALeu/control RNA) were generated through in vivo microbial fermentation production, as previously described.17,29,30 Briefly, Jo urn al Pr e-p roo f following the heterologous overexpression, BioRNAs were purified through anion exchange chromatography using an ENrich Q 10 × 100 mm column (Bio-Rad; Cat# 7800003) on an NGC Quest 10 Plus fast protein liquid chromatography (FPLC) system (Bio-Rad). .. After validated by urea polyacrylamide gel electrophoresis, FPLC fractions containing target BioRNAs were pooled, precipitated with ethanol, and subsequently desalted and concentrated by using Amicon Ultra centrifugal filters (2 mL, 30 kDa cutoff, EMD Millipore, Cat# UFC203024).

    Article Title: Calreticulin-Mediated Quality Control of the Non-Classical MHC-I Molecule MICA: Implications for Immune Surveillance.
    Article Snippet: Human CRT, coding for residues 18–417, was cloned into the pET-15b vector (Novagen, Madison, WI, USA). .. Both vectors were transformed in E. coli BL21 DE3 and purified in agarose Ni-NTA columns (Invitrogen, Carlsbad, CA, USA) and via anion exchange chromatography (UNO Q-6 Biochromatography column, Bio-Rad, Hercules, CA, USA), as previously described [35,36]. ..

    Chromatography:

    Article Title: Calreticulin-Mediated Quality Control of the Non-Classical MHC-I Molecule MICA: Implications for Immune Surveillance
    Article Snippet: Human CRT, coding for residues 18–417, was cloned into the pET-15b vector (Novagen, Madison, WI, USA). .. Both vectors were transformed in E. coli BL21 DE3 and purified in agarose Ni-NTA columns (Invitrogen, Carlsbad, CA, USA) and via anion exchange chromatography (UNO Q-6 Biochromatography column, Bio-Rad, Hercules, CA, USA), as previously described [ , ]. ..

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Cell numbers were determined using the Countess II FL automated cell counter (Life Technologies). .. The human leucyl transfer RNA fused hsa-pre-miR-34a carrier-based miR-148a-3p (BioRNALeu/miR-148a-3p) (Fig. 1A) and the corresponding control RNA (BioRNALeu/control RNA) were generated through in vivo microbial fermentation production, as previously described.17,29,30 Briefly, Jo urn al Pr e-p roo f following the heterologous overexpression, BioRNAs were purified through anion exchange chromatography using an ENrich Q 10 × 100 mm column (Bio-Rad; Cat# 7800003) on an NGC Quest 10 Plus fast protein liquid chromatography (FPLC) system (Bio-Rad). .. After validated by urea polyacrylamide gel electrophoresis, FPLC fractions containing target BioRNAs were pooled, precipitated with ethanol, and subsequently desalted and concentrated by using Amicon Ultra centrifugal filters (2 mL, 30 kDa cutoff, EMD Millipore, Cat# UFC203024).

    Article Title: A Novel Cobalt-Activated Halotolerant α-Amylase with High Specific Activity from Priestia sp. W243 in Kuwait Sabkha for Biotechnological Applications
    Article Snippet: .. The concentrated crude enzyme was then subjected to anion-exchange chromatography by fast protein liquid chromatography (FPLC) (NGC DiscoverTM 100, Bio-Rad, Hercules, CA, USA). .. The column used was ForesightTM NuviaTM Q (5 mL, Bio-Rad, Herules, CA, USA).

    Article Title: Chromium mobilisation by ligand complexation in oxic and anoxic environments and the isotopic fingerprint
    Article Snippet: .. The Cr was first separated from the Fe by anion exchange chromatography (~2mL of BioRad AG1-X8 resin loaded in a BioRad Poly-Prep column). ..

    Article Title: Calreticulin-Mediated Quality Control of the Non-Classical MHC-I Molecule MICA: Implications for Immune Surveillance.
    Article Snippet: Human CRT, coding for residues 18–417, was cloned into the pET-15b vector (Novagen, Madison, WI, USA). .. Both vectors were transformed in E. coli BL21 DE3 and purified in agarose Ni-NTA columns (Invitrogen, Carlsbad, CA, USA) and via anion exchange chromatography (UNO Q-6 Biochromatography column, Bio-Rad, Hercules, CA, USA), as previously described [35,36]. ..

    Article Title: Quantitative identification of anthropogenic sources of metal(loid)s in river sediments from a small watershed based on APCS-MLR and MixSIAR model.
    Article Snippet: Sediment contamination by metal(loid)s poses significant ecological risks.. This study employed an integrated approach, including the enrichment factor method (EF), principal component analysis (PCA), absolute principal component score-multiple linear regression (APCS-MLR), and mixing stable isotope analysis in R (MixSIAR), to investigate the spatiotemporal distribution and anthropogenic sources of six metal(loid)s (Cu, Zn, Cr, Pb, Cd, As) in a small river watershed sediment.. The findings revealed varying degrees of anthropogenic influence across the watershed.

    Article Title: A Novel Cobalt-Activated Halotolerant α-Amylase with High Specific Activity from Priestia sp. W243 in Kuwait Sabkha for Biotechnological Applications
    Article Snippet: .. The concentrated crude enzyme was then subjected to anion-exchange chromatography by fast protein liquid chromatography (FPLC) (NGC DiscoverTM 100, Bio-Rad, Hercules, CA, USA). .. The column used was ForesightTM NuviaTM Q (5 mL, Bio-Rad, Herules, CA, USA). https://doi.org/10.3390/md24020065 It was initially equilibrated with 25 mL of 25 mM Tris-HCl buffer, at a pH of 9.1 (Buffer A), with a flow rate of 5 mL/min.

    Article Title: The Discovery of Endo-Fucanases in the GH141 Family: A Novel Functional Activity Within the Family
    Article Snippet: .. The Wf141_2 was fractionated by anion exchange chromatography on a DEAE-MacroPrep column (Bio-Rad, Hercules, CA, USA) (L × W, 10 × 1.5 cm) with a linear gradient of 0–1 M NaCl in 0.04 M Na-citric buffer, pH 6.9; fractions containing the target protein were pooled, desalted, concentrated, and stored at −20 °C. ..

    Control:

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Cell numbers were determined using the Countess II FL automated cell counter (Life Technologies). .. The human leucyl transfer RNA fused hsa-pre-miR-34a carrier-based miR-148a-3p (BioRNALeu/miR-148a-3p) (Fig. 1A) and the corresponding control RNA (BioRNALeu/control RNA) were generated through in vivo microbial fermentation production, as previously described.17,29,30 Briefly, Jo urn al Pr e-p roo f following the heterologous overexpression, BioRNAs were purified through anion exchange chromatography using an ENrich Q 10 × 100 mm column (Bio-Rad; Cat# 7800003) on an NGC Quest 10 Plus fast protein liquid chromatography (FPLC) system (Bio-Rad). .. After validated by urea polyacrylamide gel electrophoresis, FPLC fractions containing target BioRNAs were pooled, precipitated with ethanol, and subsequently desalted and concentrated by using Amicon Ultra centrifugal filters (2 mL, 30 kDa cutoff, EMD Millipore, Cat# UFC203024).

    Generated:

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Cell numbers were determined using the Countess II FL automated cell counter (Life Technologies). .. The human leucyl transfer RNA fused hsa-pre-miR-34a carrier-based miR-148a-3p (BioRNALeu/miR-148a-3p) (Fig. 1A) and the corresponding control RNA (BioRNALeu/control RNA) were generated through in vivo microbial fermentation production, as previously described.17,29,30 Briefly, Jo urn al Pr e-p roo f following the heterologous overexpression, BioRNAs were purified through anion exchange chromatography using an ENrich Q 10 × 100 mm column (Bio-Rad; Cat# 7800003) on an NGC Quest 10 Plus fast protein liquid chromatography (FPLC) system (Bio-Rad). .. After validated by urea polyacrylamide gel electrophoresis, FPLC fractions containing target BioRNAs were pooled, precipitated with ethanol, and subsequently desalted and concentrated by using Amicon Ultra centrifugal filters (2 mL, 30 kDa cutoff, EMD Millipore, Cat# UFC203024).

    In Vivo:

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Cell numbers were determined using the Countess II FL automated cell counter (Life Technologies). .. The human leucyl transfer RNA fused hsa-pre-miR-34a carrier-based miR-148a-3p (BioRNALeu/miR-148a-3p) (Fig. 1A) and the corresponding control RNA (BioRNALeu/control RNA) were generated through in vivo microbial fermentation production, as previously described.17,29,30 Briefly, Jo urn al Pr e-p roo f following the heterologous overexpression, BioRNAs were purified through anion exchange chromatography using an ENrich Q 10 × 100 mm column (Bio-Rad; Cat# 7800003) on an NGC Quest 10 Plus fast protein liquid chromatography (FPLC) system (Bio-Rad). .. After validated by urea polyacrylamide gel electrophoresis, FPLC fractions containing target BioRNAs were pooled, precipitated with ethanol, and subsequently desalted and concentrated by using Amicon Ultra centrifugal filters (2 mL, 30 kDa cutoff, EMD Millipore, Cat# UFC203024).

    Over Expression:

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Cell numbers were determined using the Countess II FL automated cell counter (Life Technologies). .. The human leucyl transfer RNA fused hsa-pre-miR-34a carrier-based miR-148a-3p (BioRNALeu/miR-148a-3p) (Fig. 1A) and the corresponding control RNA (BioRNALeu/control RNA) were generated through in vivo microbial fermentation production, as previously described.17,29,30 Briefly, Jo urn al Pr e-p roo f following the heterologous overexpression, BioRNAs were purified through anion exchange chromatography using an ENrich Q 10 × 100 mm column (Bio-Rad; Cat# 7800003) on an NGC Quest 10 Plus fast protein liquid chromatography (FPLC) system (Bio-Rad). .. After validated by urea polyacrylamide gel electrophoresis, FPLC fractions containing target BioRNAs were pooled, precipitated with ethanol, and subsequently desalted and concentrated by using Amicon Ultra centrifugal filters (2 mL, 30 kDa cutoff, EMD Millipore, Cat# UFC203024).

    Fast Protein Liquid Chromatography:

    Article Title: Bioengineered miR-148a-3p suppresses glycolysis and amino acid homeostasis in hepatocellular carcinoma cells by regulating multiple solute carrier transporters
    Article Snippet: Cell numbers were determined using the Countess II FL automated cell counter (Life Technologies). .. The human leucyl transfer RNA fused hsa-pre-miR-34a carrier-based miR-148a-3p (BioRNALeu/miR-148a-3p) (Fig. 1A) and the corresponding control RNA (BioRNALeu/control RNA) were generated through in vivo microbial fermentation production, as previously described.17,29,30 Briefly, Jo urn al Pr e-p roo f following the heterologous overexpression, BioRNAs were purified through anion exchange chromatography using an ENrich Q 10 × 100 mm column (Bio-Rad; Cat# 7800003) on an NGC Quest 10 Plus fast protein liquid chromatography (FPLC) system (Bio-Rad). .. After validated by urea polyacrylamide gel electrophoresis, FPLC fractions containing target BioRNAs were pooled, precipitated with ethanol, and subsequently desalted and concentrated by using Amicon Ultra centrifugal filters (2 mL, 30 kDa cutoff, EMD Millipore, Cat# UFC203024).

    Article Title: A Novel Cobalt-Activated Halotolerant α-Amylase with High Specific Activity from Priestia sp. W243 in Kuwait Sabkha for Biotechnological Applications
    Article Snippet: .. The concentrated crude enzyme was then subjected to anion-exchange chromatography by fast protein liquid chromatography (FPLC) (NGC DiscoverTM 100, Bio-Rad, Hercules, CA, USA). .. The column used was ForesightTM NuviaTM Q (5 mL, Bio-Rad, Herules, CA, USA).

    Article Title: A Novel Cobalt-Activated Halotolerant α-Amylase with High Specific Activity from Priestia sp. W243 in Kuwait Sabkha for Biotechnological Applications
    Article Snippet: .. The concentrated crude enzyme was then subjected to anion-exchange chromatography by fast protein liquid chromatography (FPLC) (NGC DiscoverTM 100, Bio-Rad, Hercules, CA, USA). .. The column used was ForesightTM NuviaTM Q (5 mL, Bio-Rad, Herules, CA, USA). https://doi.org/10.3390/md24020065 It was initially equilibrated with 25 mL of 25 mM Tris-HCl buffer, at a pH of 9.1 (Buffer A), with a flow rate of 5 mL/min.



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